ACTH anticorps (AA 1-24)

N° du produit ABIN3025416

L’anticorps Souris Monoclonal anti-ACTH a été validé pour IF, FACS, ELISA et IHC (p). Il convient pour détecter ACTH dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour ACTH anticorps (AA 1-24) (ABIN3025416)

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ACTH est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2F6

Détail du produit anti-ACTH anticorps

Épitope: AA 1-24

Purification: Protein G affinity chromatography

Immunogène: A synthetic peptide corresponding to aa 1-24 of human ACTH was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin-fixed tissues.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur ACTH

Antigène: ACTH (Adrenocorticotropic hormone (ACTH))

Autre désignation: ACTH / Synacthen

Classe de substances: Hormone

Sujet: POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific post-translational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH (also called Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This antibody is specific to Synacthen (aa 1-24 of ACTH) and does not react with CLIP (aa 17-39 of ACTH). ACTH is produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. ACTH antibody is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

ID gène: 5443

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity