L1 anticorps
Aperçu rapide pour L1 anticorps (ABIN3025473)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Attributs du produit
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Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]
The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
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Purification
- Protein G affinity chromatography
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Immunogène
- Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the L1 antibody.
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Isotype
- IgG2a kappa
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anti-L1 antibody
Reactivité: Human Papillomavirus 16 (HPV-16) ELISA, IF, IHC (p), WB Hôte: Souris Monoclonal CamVir-1 unconjugated
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Indications d'application
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Optimal dilution of the L1 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL,ELISA: assay dependent,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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Restrictions
- For Research Use only
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Concentration
- 0.2 mg/mL
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Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store the L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- L1
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Sujet
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Reacts with a protein of 57 kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2. [UniProt]
The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33, very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
Antigène
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