ODC1 anticorps

N° du produit ABIN3025563

L’anticorps Souris Monoclonal anti-ODC1 a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter ODC1 dans des échantillons de Humain et Rat.

Aperçu rapide pour ODC1 anticorps (ABIN3025563)

Antigène: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ODC1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ODC1-487

Détail du produit anti-ODC1 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the Ornithine Decarboxylase antibody.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal dilution of the Ornithine Decarboxylase antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Ornithine Decarboxylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur ODC1

Antigène: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Autre désignation: Ornithine Decarboxylase

Sujet: Recognizes a 53  kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.