CD5 anticorps

N° du produit ABIN3025590

L’anticorps Souris Monoclonal anti-CD5 a été validé pour FACS, WB, IF et IHC (p). Il convient pour détecter CD5 dans des échantillons de Humain, Rat, Boeuf (Vache), Lapin, Cheval, Porc, Singe, Poulet et Cobaye.

Aperçu rapide pour CD5 anticorps (ABIN3025590)

Antigène: CD5

Reactivité: Humain, Rat, Boeuf (Vache), Lapin, Cheval, Porc, Singe, Poulet, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CD5-54-F6

Détail du produit anti-CD5 anticorps

Purification: Protein G affinity chromatography

Immunogène: A peptide from the intracellular region of the human protein was used as the immunogen for this CD5 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The optimal dilution of the CD5 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD5

Antigène: CD5

Autre désignation: CD5

Sujet: Recognizes a 67  kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.