CD176 anticorps

N° du produit ABIN3025630

Détail du produit anti-CD176 anticorps

Antigène: CD176 (Thomsen Friedenreich Antigen (CD176))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD176 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: PEG precipitation

Immunogène: Neuraminidase-treated human red blood cells were used as the immunogen for the Thomsen-Friedenreich Antigen antibody.

Clone: A68-B-A11

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the Thomsen-Friedenreich Antigen antibody should be determined by the researcher.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Thomsen-Friedenreich Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD176

Antigène: CD176 (Thomsen Friedenreich Antigen (CD176))

Autre désignation: Thomsen-Friedenreich Antigen (CD176 Produits)

Sujet: Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.