Nuclear Antigen anticorps

N° du produit ABIN3025764

L’anticorps Souris Monoclonal anti-Nuclear Antigen a été validé pour FACS, IF et IHC (p). Il convient pour détecter Nuclear Antigen dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Nuclear Antigen anticorps (ABIN3025764)

Antigène: Nuclear Antigen (IPO-38)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nuclear Antigen est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IPO-38

Détail du produit anti-Nuclear Antigen anticorps

Purification: PEG precipitation

Immunogène: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO-38 antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the IPO-38 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the IPO-38 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Nuclear Antigen

Antigène: Nuclear Antigen (IPO-38)

Autre désignation: IPO-38

Sujet: Recognizes a protein of 14-16  kDa, which is a novel nuclear antigen of proliferating cells. IPO38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO38 may be a useful marker of cell proliferation during monitoring of tumor progression.