Liver Arginase anticorps (AA 1-150)

N° du produit ABIN3025824

L’anticorps Souris Monoclonal anti-Liver Arginase a été validé pour WB, FACS, IF et IHC (p). Il convient pour détecter Liver Arginase dans des échantillons de Humain.

Aperçu rapide pour Liver Arginase anticorps (AA 1-150) (ABIN3025824)

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Liver Arginase est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ARG1-1125

Détail du produit anti-Liver Arginase anticorps

Épitope: AA 1-150

Purification: Protein G affinity chromatography

Immunogène: A recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.

Isotype: IgG3 kappa

Information d'application

Indications d'application: Optimal dilution of the Arginase 1 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Arginase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Liver Arginase

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Autre désignation: Arginase 1

Sujet: Recognizes a protein of 35-38  kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Pathways: Cellular Response to Molecule of Bacterial Origin