CD99 anticorps

N° du produit ABIN3025995

L’anticorps anti-CD99 Monoclonal Souris est utilisé pour la détection de CD99 dans des échantillons de Humain. Il a été validé pour FACS, IF et IHC (p).

Aperçu rapide pour CD99 anticorps (ABIN3025995)

Antigène: CD99

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD99 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 12E7-MIC2-877

Détail du produit anti-CD99 anticorps

Fonction: CD99 Antibody Cocktail

Purification: Unpurified high titer supernatant

Immunogène: Human acute lymphocytic leukemia T-cells (12E7) and recombinant human protein (MIC2/877) were used as the immunogen for the CD99 antibody cocktail.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal dilution of the CD99 antibody cocktail should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Bioreactor concentrate with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD99 antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur CD99

Antigène: CD99

Autre désignation: CD99

Sujet: Recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing's sarcoma cells and primitive peripheral neuroectodermal tumors.

UniProt: P14209