Blood Group H anticorps

N° du produit ABIN3026085

L’anticorps anti-Blood Group H Monoclonal Souris est utilisé pour la détection de Blood Group H dans des échantillons de Humain. Il a été validé pour IHC (p) et IF.

Aperçu rapide pour Blood Group H anticorps (ABIN3026085)

Antigène: Blood Group H

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: 19-OLE

Détail du produit

Purification: PEG precipitation

Immunogène: Mucinous colonic adenocarcinoma was used as the immunogen for the ABO antibody.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal dilution of the ABO antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the ABO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: Blood Group H

Autre désignation: ABO (Blood Group Antigen H Type 2)

Sujet: Recognizes the blood group H type 2 antigens, trisaccharide Fuc(a1-2)Gal(b1-4)GlcNAc(b1) of human origin. This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.