GNAS anticorps (AA 385-394)

N° du produit ABIN302636

L’anticorps Lapin Polyclonal anti-GNAS a été validé pour IHC (p) et EIA. Il convient pour détecter GNAS dans des échantillons de Humain, Singe et Primate.

Aperçu rapide pour GNAS anticorps (AA 385-394) (ABIN302636)

Antigène: GNAS (GNAS Complex Locus (GNAS))

Reactivité: Humain, Singe, Primate

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GNAS est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-GNAS anticorps

Épitope: AA 385-394

Specificité: This antibody detects GNAS at amino acids 385 to 394.

Purification: Protein G column

Immunogène: Synthetic peptide corresponding to amino acids 385 to 394 of human GNAS - KLH conjugated

Information d'application

Indications d'application: Immunohistochemistry on paraffin sections (20 μg/mL). ELISA (1: 0 - 1: 1000). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C for longer.

Détails sur GNAS

Antigène: GNAS (GNAS Complex Locus (GNAS))

Autre désignation: GNAS1 / XLalphas

Sujet: The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript exists, and this antisense transcript and one of the transcripts are paternally expressed, produce noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.Synonyms: Adenylate cyclase-stimulating G alpha protein, Extra large alphas protein, G protein alpha XLas, Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas

ID gène: 2778

NCBI Accession: NP_536350

UniProt: Q5JWF2

Pathways: Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis