HCG beta anticorps

N° du produit ABIN3026412

L’anticorps Souris Monoclonal anti-HCG beta a été validé pour ELISA et IHC (p). Il convient pour détecter HCG beta dans des échantillons de Humain.

Aperçu rapide pour HCG beta anticorps (ABIN3026412)

Antigène: HCG beta

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HCG beta est non-conjugé

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HCGb-54

Détail du produit anti-HCG beta anticorps

Attributs du produit: This antibody reacts with a protein of 22 kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

Purification: Protein G affinity chromatography

Immunogène: Recombinant protein was used as the immunogen for this HCG-beta antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HCG-beta antibody to be titered up or down for optimal performance.

1. Clone HCGb/54 (recommended detect) will pair with clones < a href=../tds/hcg-beta-antibody-hcgb211-v2374>HCGb/211 (recommended capture) and < a href=../tds/hcg-beta-antibody-hcgb459-v2373>HCGb/459 by ELISA.
2. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: use BSA-free format (1),IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (2),Prediluted format : incubate for 30 min at RT (3)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the HCG-beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur HCG beta

Antigène: HCG beta

Autre désignation: HCG-beta

Sujet: This antibody reacts with a protein of 22  kDa, identified as beta subunit of HCG. It does not cross react with the alpha subunit. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH). HCG-beta antibody detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate antibody positivity in 90 % and 60 % of cases respectively, 20 % of lung squamous cell carcinomas are positive. HCG expression by non-trophoblastic tumors may indicate aggressive behavior.

ID gène: 1082