MLANA anticorps

N° du produit ABIN3026558

Cet anticorps Souris Monoclonal détecte spécifiquement MLANA dans IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour MLANA anticorps (ABIN3026558)

Antigène: MLANA (Melan A (MLANA))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLANA est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: M2-9E3

Détail du produit anti-MLANA anticorps

Attributs du produit: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Purification: Protein G affinity chromatography

Immunogène: Recombinant human Melan-A protein was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Melan-A antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Melan-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur MLANA

Antigène: MLANA (Melan A (MLANA))

Autre désignation: Melan-A

Sujet: This antibody recognizes a protein doublet of 20-22  kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

ID gène: 2315