Actin, Muscle Specific anticorps

N° du produit ABIN3026680

Cet anticorps anti-Actin, Muscle Specific est un anticorps Souris Monoclonal détectant Actin, Muscle Specific dans FACS, IF et IHC (p). Adapté pour Humain, Lapin et Rat.

Aperçu rapide pour Actin, Muscle Specific anticorps (ABIN3026680)

Antigène: Actin, Muscle Specific

Reactivité: Humain, Lapin, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin, Muscle Specific est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM160

Détail du produit anti-Actin, Muscle Specific anticorps

Attributs du produit: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Purification: Protein G affinity chromatography

Immunogène: Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Muscle Specific Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Actin, Muscle Specific

Antigène: Actin, Muscle Specific

Autre désignation: Muscle Specific Actin

Sujet: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).