NEFH anticorps

N° du produit ABIN3026888

Cet anticorps Souris Monoclonal détecte spécifiquement NEFH dans FACS et IHC (p). Il présente une réactivité envers Humain, Rat, Souris, Poulet, Porc, Boeuf (Vache), Lapin, Cobaye, Gerbille et Cat.

Aperçu rapide pour NEFH anticorps (ABIN3026888)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Porc, Boeuf (Vache), Lapin, Cobaye, Gerbille, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NE14

Détail du produit anti-NEFH anticorps

Attributs du produit: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Purification: Protein G affinity chromatography

Immunogène: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Isotype: IgG1

Information d'application

Indications d'application: Optimal dilution of the NF-H antibody (phospho) should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the NF-H antibody (phospho) at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: NF-H

Sujet: This mAb reacts with a 200  kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.