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Secretory Component Glycoprotein anticorps

L’anticorps Souris Monoclonal anti-Secretory Component Glycoprotein a été validé pour IHC (p), IF et FACS. Il convient pour détecter Secretory Component Glycoprotein dans des échantillons de Humain et Rat.
N° du produit ABIN3026937

Aperçu rapide pour Secretory Component Glycoprotein anticorps (ABIN3026937)

Antigène

Secretory Component Glycoprotein

Reactivité

Humain, Rat

Hôte

  • 3
Souris

Clonalité

  • 3
Monoclonal

Conjugué

  • 3
Cet anticorp Secretory Component Glycoprotein est non-conjugé

Application

  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

SPM217
  • Attributs du produit

    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

    Purification

    Protein G affinity chromatography

    Immunogène

    Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.

    Isotype

    IgG1 kappa
  • Indications d'application

    The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Agent conservateur

    Azide free

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store the Secretory Component antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Antigène

    Secretory Component Glycoprotein

    Sujet

    This mAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
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