HLA-Aw32-&-HLA-A25 (MHC-I) anticorps

N° du produit ABIN3026979

L’anticorps anti-HLA-Aw32-&-HLA-A25 (MHC-I) Monoclonal Souris est utilisé pour la détection de HLA-Aw32-&-HLA-A25 (MHC-I) dans des échantillons de Humain. Il a été validé pour FACS, IF et IHC (p).

Aperçu rapide pour HLA-Aw32-&-HLA-A25 (MHC-I) anticorps (ABIN3026979)

Antigène: HLA-Aw32-&-HLA-A25 (MHC-I)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: Carrier-free

Clone: CATA-1

Détail du produit

Fonction: HLA-A25 Antibody (HLA-Aw32) (azide and preservative free)

Attributs du produit: This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

Purification: Protein G affinity chromatography

Immunogène: Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, and DRw10 were used as the immunogen for the HLA-A25 antibody (HLA-Aw32).

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal dilution of the HLA-A25 antibody (HLA-Aw32) should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the HLA-A25 antibody (HLA-Aw32) at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détail du antigène

Antigène: HLA-Aw32-&-HLA-A25 (MHC-I)

Autre désignation: HLA-A25 (HLA-Aw32)

Sujet: This mAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This mAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

UniProt: P30443