Cullin 4B anticorps (C-Term)

N° du produit ABIN3030579

Cet anticorps anti-Cullin 4B est un anticorps Lapin Polyclonal détectant Cullin 4B dans WB et IHC (p). Adapté pour Humain, Souris, Rat et Poisson zèbre (Danio rerio).

Aperçu rapide pour Cullin 4B anticorps (C-Term) (ABIN3030579)

Antigène: Cullin 4B (CUL4B)

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cullin 4B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cullin 4B anticorps

Épitope: C-Term

Purification: Antigen affinity

Immunogène: An amino acid sequence from the C-terminus of human Cullin 4B (DRDYMERDKENPNQYNYIA) was used as the immunogen for this Cullin 4B antibody (100% homologous in human, mouse and rat).

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the Cullin 4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: -20 °C

Stockage commentaire: After reconstitution, the Cullin 4B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur Cullin 4B

Antigène: Cullin 4B (CUL4B)

Autre désignation: Cullin 4B

Sujet: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING(really interesting new gene) ubiquitin ligase(E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain(cullin homology domain) and a series of N-terminal repeats(cullin repeats). Ohtake et al.(2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor(AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B(AhR). Complex assembly and ubiquitin ligase activity of CUL4B(AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B(AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

UniProt: Q13620