DCI anticorps (C-Term)

N° du produit ABIN3030698

L’anticorps anti-DCI Polyclonal Lapin est utilisé pour la détection de DCI dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, FACS et IHC (p).

Aperçu rapide pour DCI anticorps (C-Term) (ABIN3030698)

Antigène: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DCI est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DCI anticorps

Épitope: C-Term

Fonction: DCI Antibody / Dodecenoyl-CoA Delta Isomerase / ECI1

Séquence: ADVQNFVSFI SKDSIQKSL

Purification: Antigen affinity

Immunogène: An amino acid sequence from the C-terminus of human DCI (ADVQNFVSFISKDSIQKSL) was used as the immunogen for this DCI antibody.

Isotype: IgG

Information d'application

Indications d'application: The stated application concentrations are suggested starting amounts. Titration of the DCI antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the DCI antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur DCI

Antigène: DCI (ECI1) (Enoyl-CoA Delta Isomerase 1 (ECI1))

Autre désignation: DCI

Sujet: DCI Antibody targets Dodecenoyl-CoA delta isomerase, encoded by the ECI1 gene. Dodecenoyl-CoA delta isomerase is a mitochondrial enzyme that plays a critical role in the beta-oxidation of unsaturated fatty acids. This enzyme catalyzes the isomerization of cis- or trans-unsaturated enoyl-CoA intermediates, enabling their continued processing through the mitochondrial fatty acid degradation pathway. As such, Dodecenoyl-CoA delta isomerase serves as an essential auxiliary enzyme that ensures efficient energy production from dietary and stored lipids.

Functionally, Dodecenoyl-CoA delta isomerase acts downstream of acyl-CoA dehydrogenases and enoyl-CoA hydratases, converting specific unsaturated fatty acyl-CoA substrates into configurations compatible with the core beta-oxidation machinery. Without this isomerization step, certain unsaturated fatty acids would accumulate as metabolic dead ends, reducing overall oxidative capacity. A DCI Antibody is therefore useful for studying mitochondrial lipid metabolism, energy homeostasis, and the regulation of fatty acid utilization under physiological and stress conditions.

Expression of Dodecenoyl-CoA delta isomerase is observed in many metabolically active tissues, including liver, muscle, heart, and kidney, reflecting the widespread requirement for fatty acid oxidation. Subcellular localization is predominantly mitochondrial, consistent with its enzymatic role within the beta-oxidation pathway. The protein contains a conserved catalytic domain characteristic of enoyl-CoA isomerases, supporting substrate binding and stereochemical conversion of fatty acyl-CoA intermediates. Studies often examine ECI1 localization and abundance to better understand mitochondrial function and metabolic flexibility.

From a disease relevance perspective, alterations in mitochondrial fatty acid oxidation pathways have been linked to metabolic disorders, insulin resistance, and inherited defects of lipid metabolism. While ECI1 is not among the most frequently mutated beta-oxidation enzymes, changes in its expression or activity may contribute to broader mitochondrial dysfunction and impaired lipid handling. Dodecenoyl-CoA delta isomerase has also been examined in the context of cancer metabolism, where reprogramming of fatty acid oxidation can support tumor growth and survival under nutrient-limited conditions.

At the molecular level, Dodecenoyl-CoA delta isomerase functions as a soluble mitochondrial matrix protein, interacting indirectly with other beta-oxidation enzymes through coordinated metabolic flux rather than stable protein complexes. Antibody-based detection of Dodecenoyl-CoA delta isomerase supports research into mitochondrial biology, lipid metabolism, and metabolic disease mechanisms. NSJ Bioreagents provides reagents intended for research use to support investigations involving ECI1 expression and mitochondrial fatty acid oxidation pathways.

UniProt: P42126