Cullin 3 anticorps

N° du produit ABIN303084

Cet anticorps anti-Cullin 3 Polyclonal Lapin (ABIN303084) détecte spécifiquement Cullin 3 dans WB, IP, IHC (p) et EIA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Cullin 3 anticorps (ABIN303084)

Antigène: Cullin 3 (CUL3)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cullin 3 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Cullin 3 anticorps

Specificité: This antibody reacts to amino acids 1-12 of Cul3 (N-terminus) coupled to KLH.

Purification: Delipidation and defibrination

Immunogène: Synthetic peptide

Information d'application

Indications d'application: Immunoprecipitation. Immunohistochemistry on paraffin sections: 1: 500. Western Blot: 1: 500 - 1: 1000. ELISA: 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur Cullin 3

Antigène: Cullin 3 (CUL3)

Autre désignation: Cullin-3

Sujet: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.Synonyms: CUL-3, CUL3, KIAA0617

ID gène: 8452

NCBI Accession: NP_003581

UniProt: Q13618

Pathways: M Phase