Villin 1 anticorps (C-Term)

N° du produit ABIN3042343

Cet anticorps Lapin Polyclonal détecte spécifiquement Villin 1 dans IHC, WB, IF et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Villin 1 anticorps (C-Term) (ABIN3042343)

Antigène: Villin 1 (VIL1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Villin 1 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Villin 1 anticorps

Épitope: AA 770-799, C-Term

Fonction: Anti-Villin/VIL1 Antibody Picoband®

Séquence: EQLVNKPVEE LPEGVDPSRK EEHLSIEDFT

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Villin/VIL1 Antibody Picoband® (ABIN3042343). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Villin, different from the related mouse sequence by three amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Friederich, E, Vancompernolle, K, Louvard, D, Vandekerckhove, J (1999). "Villin function in the organization of the actin cytoskeleton. Correlation of in vivo effects to its biochemical activities in vitro". The Journal of Biological Chemistry274 (38): 26751-60. 2. Meng, J, Vardar, D, Wang, Y, Guo, HC, Head, JF, McKnight, CJ (2005). "High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity". Biochemistry 44 (36): 11963-73.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Villin 1

Antigène: Villin 1 (VIL1)

Autre désignation: VIL1

Sujet:

Synonyms: Villin-1,VIL1,VIL,

Tissue Specificity: Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level). .

Background: Villin is known as VIL1. This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed, they result from utilization of alternate poly-adenylation signals present in the terminal exon. In vertebrates, the villin proteins help to support the microfilaments of the microvilli of the brush border. It may play a role in cell plasticity through F-actin severing.

Sequence Similarities: Belongs to the villin/gelsolin family.

Poids moléculaire: 93 kDa

ID gène: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization