Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) anticorps

N° du produit ABIN3042370

Cet anticorps Lapin Polyclonal détecte spécifiquement dans WB, IHC, ICC, IHC (fro) et FACS. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour Crossover junction endonuclease EME1 (EME1) (AA 520-561), (C-Term) anticorps (ABIN3042370)

Antigène: Crossover junction endonuclease EME1 (EME1)

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Détail du produit

Épitope: AA 520-561, C-Term

Fonction: Anti-EME1 Antibody Picoband®

Séquence: DKERQNLLAD IQVRRGEGVT STSRRIGPEL SRRIYLQMTT LQ

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-EME1 Antibody Picoband® (ABIN3042370). Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human EME1, different from the related mouse sequence by five amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: EME1 essential meiotic endonuclease 1 homolog 1 (S. pombe)". 2. Abraham, J., Lemmers, B., Hande, M. P., Moynahan, M. E., Chahwan, C., Ciccia, A., Essers, J., Hanada, K., Chahwan, R., Khaw, A. K., McPherson, P., Shehabeldin, A., Laister, R., Arrowsmith, C., Kanaar, R., West, S. C., Jasin, M., Hakem, R. Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells. EMBO J. 22: 6137-6147, 2003. 3. Ciccia A, Constantinou A, West SC (Jun 2003). "Identification and characterization of the human mus81-eme1 endonuclease". J Biol Chem 278 (27): 25172-8.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: Crossover junction endonuclease EME1 (EME1)

Autre désignation: EME1

Sujet:

Synonyms: Crossover junction endonuclease EME1,3.1.22.-,MMS4 homolog,hMMS4,EME1,MMS4,

Tissue Specificity: Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression. .

Background: Crossover junction endonuclease EME1 is an enzyme that in humans is encoded by the EME1 gene. It is mapped to 17q21.33. This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. Also, this protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.

Poids moléculaire: 62 kDa

ID gène: 146956