CD20 anticorps (AA 1-261)

N° du produit ABIN3042388

Cet anticorps Lapin Polyclonal détecte spécifiquement CD20 dans FACS, WB, IHC, IF, IHC (fro) et ICC. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour CD20 anticorps (AA 1-261) (ABIN3042388)

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD20 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Détail du produit anti-CD20 anticorps

Épitope: AA 1-261

Fonction: Anti-CD20/MS4A1 Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-CD20/MS4A1 Antibody (ABIN3042388). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶cells

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-CD20 (ABIN3042388)

Ni, Chen, Zeng, Pei, Du, Tang, Wang, Hu, Zhu, He, Wei, Wang, Ning, Wang, Wang, Ma, Chen, Sun, Tang, Wang, Wang: "In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures." dans: Cell research, Vol. 25, Issue 7, pp. 785-800, (2016) (PubMed).

Yan, Fan, Bai, Hou, Li, Tong: "Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-κB Pathway." dans: PLoS ONE, Vol. 11, Issue 2, pp. e0148536, (2016) (PubMed).

Détails sur CD20

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Autre désignation: MS4A1

Sujet:

Synonyms: B-lymphocyte antigen CD20,B-lymphocyte surface antigen B1,Bp35,Leukocyte surface antigen Leu-16,Membrane-spanning 4-domains subfamily A member 1,CD20,MS4A1,CD20,

Tissue Specificity: Expressed on B-cells.

Background: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What's more, this protein may be involved in the regulation of B-cell activation and proliferation.

Sequence Similarities: Belongs to the MS4A family.

Poids moléculaire: 35 kDa

ID gène: 931

UniProt: P11836