FHIT anticorps (AA 1-147)

N° du produit ABIN3042392

Il existe 2+ publications pour ce produit. L’anticorps anti-FHIT Polyclonal Lapin est utilisé pour la détection de FHIT dans des échantillons de Humain et Rat. Il a été validé pour WB, IHC, IF, FACS et ICC.

Aperçu rapide pour FHIT anticorps (AA 1-147) (ABIN3042392)

Antigène: FHIT (Fragile Histidine Triad (FHIT))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FHIT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-FHIT anticorps

Épitope: AA 1-147

Fonction: Anti-FHIT Antibody

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: Human FHIT shares 90%,87% amino acid (aa) sequences identity with mouse,rat FHIT,respectively.

Attributs du produit: Anti-FHIT Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequences identity with mouse and rat FHIT, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-FHIT (ABIN3042392)

Li, Li, Xu, Guo, Du: "Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia." dans: International journal of molecular sciences, Vol. 11, Issue 11, pp. 4580-90, (2010) (PubMed).

Tracy, Liu, Chapman: "Coxsackievirus B3: primary structure of the 5' non-coding and capsid protein-coding regions of the genome." dans: Virus research, Vol. 3, Issue 3, pp. 263-70, (1986) (PubMed).

Détails sur FHIT

Antigène: FHIT (Fragile Histidine Triad (FHIT))

Autre désignation: FHIT

Sujet:

Background: Bis (5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis (5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.

Gene Full Name: fragile histidine triad diadenosine triphosphatase

Sequence Similarities: Contains 1 HIT domain.

Poids moléculaire: 17 kDa

ID gène: 2272

UniProt: P49789