FHIT anticorps (AA 1-147)

N° du produit ABIN3042392

Détail du produit anti-FHIT anticorps

Antigène: FHIT (Fragile Histidine Triad (FHIT))

Épitope: AA 1-147

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FHIT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Bis(5'-adenosyl)-triphosphatase(FHIT) detection. Tested with WB, IHC-P in Human.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Bis(5'-adenosyl)-triphosphatase(FHIT) detection. Tested with WB, IHC-P in Human.
Gene Name: fragile histidine triad
Protein Name: Bis(5'-adenosyl)-triphosphatase

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequences identity with mouse and rat FHIT, respectively.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for FHIT is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-FHIT (ABIN3042392)

Li, Li, Xu, Guo, Du: "Neuroprotective properties of picroside II in a rat model of focal cerebral ischemia." dans: International journal of molecular sciences, Vol. 11, Issue 11, pp. 4580-90, (2010) (PubMed).

Tracy, Liu, Chapman: "Coxsackievirus B3: primary structure of the 5' non-coding and capsid protein-coding regions of the genome." dans: Virus research, Vol. 3, Issue 3, pp. 263-70, (1986) (PubMed).

Détails sur FHIT

Antigène: FHIT (Fragile Histidine Triad (FHIT))

Autre désignation: FHIT (FHIT Produits)

Synonymes: anticorps AP3Aase, anticorps FRA3B, anticorps FHIT, anticorps zgc:73176, anticorps AW045638, anticorps Fra14A2, anticorps LOC100223655, anticorps fragile histidine triad, anticorps fragile histidine triad gene, anticorps fragile histidine triad protein, anticorps fragile histidine triad L homeolog, anticorps FHIT, anticorps Fhit, anticorps fhit, anticorps PY07476, anticorps fhit.L

Sujet: Bis(5'-adenosyl)-triphosphatase, also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by the FHIT gene. This gene, a member of the histidine triad gene family, encodes a diadenosine P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3p14.2, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Furthermore, FHIT has been shown to synergize with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. It also acts as a tumor suppressor of HER2/neu driven breast cancer.

Synonyms: AP3A hydrolase antibody|AP3A hydrolase fragile site 3p14.2 antibody|AP3Aase antibody|Bis 5' adenosyl triphosphatase antibody|Bis(5' adenosyl) triphosphatase antibody|Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody|Dinucleosidetriphosphatase antibody|FRA 3B antibody|FRA3B antibody|Fragile histidine triad antibody|Fragile histidine triad gene antibody|Fragile histidine triad protein antibody|Tumor suppressor protein antibody

ID gène: 2272

UniProt: P49789