OPTN anticorps (AA 241-577)

N° du produit ABIN3042498

Cet anticorps anti-OPTN est un anticorps Lapin Polyclonal détectant OPTN dans WB, IHC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour OPTN anticorps (AA 241-577) (ABIN3042498)

Antigène: OPTN (Optineurin (OPTN))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OPTN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-OPTN anticorps

Épitope: AA 241-577

Fonction: Anti-Optineurin/OPTN Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Optineurin/OPTN Antibody Picoband® (ABIN3042498). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Optineurin recombinant protein (Position: R241-I577). Human Optineurin shares 82% amino acid (aa) sequence identity with both mouse and rat Optineurin.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Johnson L, et al. Screening for OPTN mutations in a cohort of British amyotrophic lateral sclerosis patients. Neurobiol Aging, 2012 Dec. 2. Michou L, et al. Genetic association study of UCMA/GRP and OPTN genes (PDB6 locus) with Paget's disease of bone. Bone, 2012 Oct. 3. Weishaupt JH, et al. A novel optineurin truncating mutation and three glaucoma-associated missense variants in patients with familial amyotrophic lateral sclerosis in Germany. Neurobiol Aging, 2013 May.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur OPTN

Antigène: OPTN (Optineurin (OPTN))

Autre désignation: OPTN

Sujet:

Synonyms: Optineurin,E3-14.7K-interacting protein,FIP-2,Huntingtin yeast partner L,Huntingtin-interacting protein 7,HIP-7,Huntingtin-interacting protein L,NEMO-related protein,Optic neuropathy-inducing protein,Transcription factor IIIA-interacting protein,TFIIIA-IntP,OPTN,FIP2, GLC1E, HIP7, HYPL, NRP,

Tissue Specificity: Present in aqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta. .

Background: OPTN is also known as NRP, FIP2 or HYPL. This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.

Sequence Similarities: Belongs to the nuclear hormone receptor family. NR3 subfamily.

Poids moléculaire: 75 kDa

ID gène: 10133

Pathways: M Phase