CARM1 anticorps (Middle Region)

N° du produit ABIN3042568

Cet anticorps Lapin Polyclonal détecte spécifiquement CARM1 dans WB, IF, FACS et ICC. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour CARM1 anticorps (Middle Region) (ABIN3042568)

Antigène: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CARM1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-CARM1 anticorps

Épitope: AA 267-284, Middle Region

Fonction: Anti-PRMT4/CARM1 Antibody Picoband®

Séquence: HLAPFTDEQL YMEQFTKA

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PRMT4/CARM1 Antibody (ABIN3042568). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human PRMT4, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chen D, Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, Stallcup MR (1999). "Regulation of transcription by a protein methyltransferase". Science 284(5423): 2174-7. 2. El Messaoudi, S. E., Fabbrizio, E., Rodriguez, C., Chuchana, P., Fauquier, L., Cheng, D., Theillet, C., Vandel, L., Bedford, M. T., Sardet, C. Coactivator-associated arginine methyltransferase 1 (CARM1) is a positive regulator of the cyclin E1 gene. Proc. Nat. Acad. Sci. 103: 13351-13356, 2006. 3. Torres-Padilla ME, Parfitt DE, Kouzarides T, Zernicka-Goetz M (2007). "Histone arginine methylation regulates pluripotency in the early mouse embryo". Nature445 (7124): 214-8.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CARM1

Antigène: CARM1 (Coactivator-Associated Arginine Methyltransferase 1 (CARM1))

Autre désignation: CARM1

Sujet:

Synonyms: Histone-arginine methyltransferase CARM1,2.1.1.-,2.1.1.125,Coactivator-associated arginine methyltransferase 1,Protein arginine N-methyltransferase 4,CARM1,PRMT4,

Tissue Specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. .

Background: CARM1 (coactivator-associated arginine methyltransferase 1), also known as PRMT4, is an enzyme encoded by the CARM1 gene found in human beings, as well as many other mammals. This gene is mapped to 19p13.2. CARM1 is a regulator of cyclin E1 and DHFR mRNA expression. Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. CARM1 is a secondary coactivator through its association with p160 family (SRC-1, GRIP1, AIB) of coactivators. It is responsible for moving cells toward the inner cell mass in developing blastocysts. This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression.

Sequence Similarities: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family.

Poids moléculaire: 66 kDa

UniProt: Q86X55

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of fat Cell Differentiation