E2F3 anticorps (C-Term)

N° du produit ABIN3042877

Cet anticorps Lapin Polyclonal détecte spécifiquement E2F3 dans WB. Il présente une réactivité envers Humain et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour E2F3 anticorps (C-Term) (ABIN3042877)

Antigène: E2F3

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F3 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-E2F3 anticorps

Épitope: AA 446-465, C-Term

Fonction: Anti-Transcription factor E2F3 E2F3 Antibody Picoband®

Séquence: DLFDAYDLEK LPLVEDFMCS

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Transcription factor E2F3 E2F3 Antibody (ABIN3042877). Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human E2F3, identical to the related mouse sequence.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. Chen, D., Pacal, M., Wenzel, P., Knoepfler, P. S., Leone, G., Bremner, R. Division and apoptosis of E2f-deficient retinal progenitors. Nature 462: 925-929, 2009. 2. Chong, J.-L., Wenzel, P. L., Saenz-Robles, M. T., Nair, V., Ferrey, A., Hagan, J. P., Gomez, Y. M., Sharma, N., Chen, H.-Z., Ouseph, M., Wang, S.-H., Trikha, P., and 10 others. E2f1-3 switch from activators in progenitor cells to repressors in differentiating cells. Nature 462: 930-934, 2009.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-E2F3 (ABIN3042877)

Xie, Li, Wang, Jiao, Zhang, Zhang: "miRNA-regulated expression of oncogenes and tumor suppressor genes in the cisplatin-inhibited growth of K562 cells." dans: Oncology reports, Vol. 23, Issue 6, pp. 1693-700, (2010) (PubMed).

Wang, Li, Zhang, Li, Yue, Xie, Xie: "Regulating A549 cells growth by ASO inhibiting miRNA expression." dans: Molecular and cellular biochemistry, Vol. 339, Issue 1-2, pp. 163-71, (2010) (PubMed).

Détails sur E2F3

Antigène: E2F3

Sujet:

Synonyms: Transcription factor E2F3,E2F-3,E2F3,KIAA0075,

Background: Transcription factor E2F3, also known as KIAA0075, is a protein that in humans is encoded by the E2F3 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. By fluorescence in situ hybridization, E2F3 gene is mapped to 6q22.3. The induction of specific E2F activities is an essential component in the MYC pathways that control cell proliferation and cell fate decisions. This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts ly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle.

Sequence Similarities: Belongs to the E2F/DP family.

Poids moléculaire: 49 kDa

UniProt: O00716