T-Bet anticorps (C-Term)

N° du produit ABIN3042957

Cet anticorps anti-T-Bet est un anticorps Lapin Polyclonal détectant T-Bet dans WB. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour T-Bet anticorps (C-Term) (ABIN3042957)

Antigène: T-Bet

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp T-Bet est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-T-Bet anticorps

Épitope: AA 522-535, C-Term

Fonction: Anti-T-bet/Tbx21 Antibody Picoband®

Séquence: DKEAEGQFYN YFPN

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-T-bet/Tbx21 Antibody (ABIN3042957). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Finotto, S., Neurath, M. F., Glickman, J. N., Qin, S., Lehr, H. A., Green, F. H. Y., Ackerman, K., Haley, K., Galle, P. R., Szabo, S. J., Drazen, J. M., De Sanctis, G. T., Glimcher, L. H. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet. Science 295: 336-338, 2002. 2. Szabo, S. J., Kim, S. T., Costa, G. L., Zhang, X., Fathman, C. G., Glimcher, L. H. A novel transcription factor, T-bet, s Th1 lineage commitment. Cell 100: 655-669, 2000. 3. Zhang, W., Yang, S. Y. Cloning and characterization of a new member of the T-box gene family. Genomics 70: 41-48, 2000.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-T-Bet (ABIN3042957)

Miao, Run-Ming, Yi: "T-Bet Mediated Anti-Neoplastic Effects of Dendritic Cell-Cytokine Induced Killer Cells in vitro." dans: Iranian journal of pediatrics, Vol. 22, Issue 1, pp. 43-51, (2012) (PubMed).

Détails sur T-Bet

Antigène: T-Bet

Autre désignation: TBX21

Sujet:

Synonyms: T-box transcription factor TBX21,T-box protein 21,T-cell-specific T-box transcription factor T-bet,Transcription factor TBLYM,TBX21,TBET, TBLYM,

Tissue Specificity: T-cell specific.

Background: TBX21 (T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang (2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al. (2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al. (2002) observed reduced expression of the T (H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Sequence Similarities: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Poids moléculaire: 58-60 kDa