PPP1R12A anticorps (N-Term)

N° du produit ABIN3043106

L’anticorps Lapin Polyclonal anti-PPP1R12A a été validé pour WB, IF et ICC. Il convient pour détecter PPP1R12A dans des échantillons de Humain, Souris, Rat et Singe. Il y a 1 publication disponible.

Aperçu rapide pour PPP1R12A anticorps (N-Term) (ABIN3043106)

Antigène: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPP1R12A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PPP1R12A anticorps

Épitope: AA 1-17, N-Term

Fonction: Anti-Myosin Phosphatase/PPP1R12A Antibody Picoband®

Séquence: MKMADAKQKR NEQLKRW

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Myosin Phosphatase/PPP1R12A Antibody (ABIN3043106). Tested in IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PPP1R12A, identical to the related rat and mouse sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Kimura, K., Ito, M., Amano, M., Chihara, K., Fukata, Y., Nakafuku, M., Yamamori, B., Feng, J., Nakano, T., Okawa, K., Iwamatsu, A., Kaibuchi, K. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 273: 245-248, 1996. 2. Takahashi, N., Ito, M., Tanaka, J., Nakano, T., Kaibuchi, K., Odai, H., Takemura, K. Localization of the gene coding for myosin phosphatase, target subunit 1 (MYPT1) to human chromosome 12q15-q21. Genomics 44: 150-152, 1997.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Thimerosal, 0.01 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-PPP1R12A (ABIN3043106)

Yan, Wang, Jiang, Chen, He, Mang, Shao, Xu: "The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia." dans: Neural regeneration research, Vol. 10, Issue 9, pp. 1441-9, (2015) (PubMed).

Détails sur PPP1R12A

Antigène: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Autre désignation: PPP1R12A

Sujet:

Synonyms: Protein phosphatase 1 regulatory subunit 12A,Myosin phosphatase-targeting subunit 1,Myosin phosphatase target subunit 1,Protein phosphatase myosin-binding subunit,PPP1R12A,MBS, MYPT1,

Tissue Specificity: Expressed in striated muscles, specifically in type 2a fibers (at protein level). .

Background: PPP1R12A (Protein phosphatase 1 regulatory subunit 12A), also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Sequence Similarities: Contains 6 ANK repeats.

Poids moléculaire: 140 kDa

UniProt: O14974

Pathways: M Phase