Lipocalin 2 anticorps (AA 21-198)

N° du produit ABIN3043291

Cet anticorps Lapin Polyclonal détecte spécifiquement Lipocalin 2 dans WB, ELISA, IHC et IHC (fro). Il présente une réactivité envers Humain, Rat et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour Lipocalin 2 anticorps (AA 21-198) (ABIN3043291)

Antigène: Lipocalin 2 (LCN2)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Lipocalin 2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Lipocalin 2 anticorps

Épitope: AA 21-198

Fonction: Anti-Lipocalin 2/LCN2 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Lipocalin 2/LCN2 Antibody Picoband® (ABIN3043291). Tested in ELISA, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
ELISA (cap) ,1-5 μg/mL, Rat
1. Cowland JB, Borregaard N (October 1997). "Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans". Genomics 45 (1): 17-23. 2. Chan P, Simon-Chazottes D, Mattei MG, Guenet JL, Salier JP (September 1994). "Comparative mapping of lipocalin genes in human and mouse: the four genes for complement C8 gamma chain, prostaglandin-D-synthase, oncogene-24p3, and progestagen-associated endometrial protein map to HSA9 and MMU2". Genomics 23 (1): 145-50. 3. Flo TH, Smith KD, Sato S, Rodriguez DJ, Holmes MA, Strong RK, Akira S, Aderem A (December 2004). "Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron". Nature 432 (7019): 917-21. 4. Kjeldsen L, Johnsen AH, Sengel111v H, Borregaard N (May 1993). "Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase". J. Biol. Chem. 268 (14): 10425-32. 5. Nelson AM, Zhao W, Gilliland KL, Zaenglein AL, Liu W, Thiboutot DM (April 2008). 6. Schmidt-Ott KM, Mori K, Li JY, Kalandadze A, Cohen DJ, Devarajan P, Barasch J (February 2007). "Dual action of neutrophil gelatinase-associated lipocalin". J. Am. Soc. Nephrol. 18 (2): 407-13.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Lipocalin 2 (ABIN3043291)

Narayan, Duan, Jiang, Li, Paka, Yamin, Friedman, Weir, Goldberg: "Late intervention with the small molecule BB3 mitigates postischemic kidney injury." dans: American journal of physiology. Renal physiology, Vol. 311, Issue 2, pp. F352-61, (2017) (PubMed).

Li, Wang, He, Chen, Yu: "Expression of neutrophil gelatinase-associated lipocalin in low osmolar contrast-induced nephropathy in rats and the effect of N-acetylcysteine." dans: Experimental and therapeutic medicine, Vol. 12, Issue 5, pp. 3175-3180, (2016) (PubMed).

Détails sur Lipocalin 2

Antigène: Lipocalin 2 (LCN2)

Autre désignation: Lcn2

Sujet:

Synonyms: Neutrophil gelatinase-associated lipocalin,NGAL,Alpha-2-microglobulin-related protein,Alpha-2U globulin-related protein,Lipocalin-2,Siderocalin LCN2,p25,Lcn2,

Tissue Specificity: Detected in lung, spleen, uterus, vagina and epididymis. .

Background: Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Sequence Similarities: Belongs to the KIN17 family.

Poids moléculaire: 22 kDa

ID gène: 170496

UniProt: P30152

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis