NUMB anticorps (N-Term)

N° du produit ABIN3043374

Cet anticorps anti-NUMB est un anticorps Lapin Polyclonal détectant NUMB dans WB, IHC et FACS. Adapté pour Humain, Souris, Rat et Singe.

Aperçu rapide pour NUMB anticorps (N-Term) (ABIN3043374)

Antigène: NUMB (Numb Homolog (NUMB))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NUMB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-NUMB anticorps

Épitope: AA 194-220, N-Term

Fonction: Anti-NUMB Antibody Picoband®

Séquence: SFRVTTATEQ AEREEIMKQM QDAKKAE

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-NUMB Antibody Picoband® (ABIN3043374). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human NUMB, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Dho SE, French MB, Woods SA, McGlade CJ (November 1999). "Characterization of four mammalian numb protein isoforms. Identification of cytoplasmic and membrane-associated variants of the phosphotyrosine binding domain". J. Biol. Chem. 274 (46): 33097-104. 2. Katoh M, Katoh M (September 2006). "NUMB is a break of WNT-Notch signaling cycle". Int. J. Mol. Med. 18(3): 517-21. 3. Pece S, Confalonieri S, R Romano P, Di Fiore PP (January 2011). "NUMB-ing down cancer by more than just a NOTCH". Biochim. Biophys. Acta 1815 (1): 26-43.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur NUMB

Antigène: NUMB (Numb Homolog (NUMB))

Autre désignation: NUMB

Sujet:

Synonyms: Protein numb homolog,h-Numb,Protein S171,NUMB,

Tissue Specificity: The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta, moderately in lung, liver, skeletal muscle, kidney and pancreas, and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Background: Protein numb homolog is a protein that in humans is encoded by the NUMB gene. It is mapped to 14q24.2 to q24.3. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. The primary function of Numb in cell differentiation is as an inhibitor of Notch signaling which is essential for maintaining self-renewal potential in stem and progenitor cells. Numb also plays a crucial role in asymmetrical cell division during development, allowing for differential cell fate specification in the central and peripheral nervous systems. What's more, the numb gene protein product controls binary cell fate decisions in the peripheral and central nervous systems of both invertebrates and mammals during neurogenesis.

Sequence Similarities: Contains 1 PID domain.

Poids moléculaire: 71 kDa

ID gène: 8650

UniProt: P49757

Pathways: Cell-Cell Junction Organization