CDC6 anticorps (AA 147-549)

N° du produit ABIN3043500

Cet anticorps anti-CDC6 est un anticorps Lapin Polyclonal détectant CDC6 dans WB et IHC. Adapté pour Humain et Rat.

Aperçu rapide pour CDC6 anticorps (AA 147-549) (ABIN3043500)

Antigène: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-CDC6 anticorps

Épitope: AA 147-549

Fonction: Anti-Cdc6 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Cdc6 Antibody Picoband® (ABIN3043500). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gonzalez, S., Klatt, P., Delgado, S., Conde, E., Lopez-Rios, F., Sanchez-Cespedes, M., Mendez, J., Antequera, F., Serrano, M. Oncogenic activity of Cdc6 through repression of the INK4/ARF locus. Nature 440: 702-706, 2006. 2. Mimura, S., Seki, T., Tanaka, S., Diffley, J. F. X. Phosphorylation-dependent binding of mitotic cyclins to Cdc6 contributes to DNA replication control. Nature 431: 1118-1123, 2004. 3. Whitmire, E., Khan, B., Coue, M. Cdc6 synthesis regulates replication competence in Xenopus oocytes. Nature 419: 722-725, 2002.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur CDC6

Antigène: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Autre désignation: CDC6

Sujet:

Synonyms: Cell division control protein 6 homolog,CDC6-related protein,Cdc18-related protein,HsCdc18,p62 (cdc6),HsCDC6,CDC6,CDC18L,

Tissue Specificity: Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.

Background: Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene. By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. And this protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. This gene is involved in the initiation of DNA replication.

Sequence Similarities: Belongs to the CDC6/cdc18 family.

Poids moléculaire: 63 kDa

ID gène: 990

UniProt: Q99741

Pathways: DNA Replication, Synthesis of DNA