PFN1 anticorps (C-Term)

N° du produit ABIN3043603

Cet anticorps Lapin Polyclonal détecte spécifiquement PFN1 dans WB, IHC, ICC, FACS et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PFN1 anticorps (C-Term) (ABIN3043603)

Antigène: PFN1 (Profilin 1 (PFN1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PFN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-PFN1 anticorps

Épitope: AA 116-140, C-Term

Fonction: Anti-Profilin 1/PFN1 Antibody Picoband®

Séquence: KEGVHGGLIN KKCYEMASHL RRSQY

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Profilin 1/PFN1 Antibody Picoband® (ABIN3043603). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Profilin 1, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: PFN1 profilin 1" 2. Kwiatkowski, D. J., Aklog, L., Ledbetter, D. H., Morton, C. C. Identification of the functional profilin gene, its localization to chromosome subband 17p13.3, and demonstration of its deletion in some patients with Miller-Dieker syndrome. Am. J. Hum. Genet. 46: 559-567, 1990. 3. Mockrin, S. C., Korn, E. D. Acanthamoeba profilin interacts with G-actin to increase the rate of exchange of actin-bound adenosine 5-prime-triphosphate. Biochemistry 19: 5359-5362, 1980.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PFN1

Antigène: PFN1 (Profilin 1 (PFN1))

Autre désignation: PFN1

Sujet:

Synonyms: Profilin-1,Epididymis tissue protein Li 184a,Profilin I,PFN1,

Tissue Specificity: Expressed in epididymis (at protein level). .

Background: Profilin-1 is a protein that in humans is encoded by the PFN1 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. PFN1 is mapped to 17p13.2. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. This gene is a 140-amino acid protein and major growth regulator of filamentous (F)-actin through its binding of monomeric (G)-actin. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.

Sequence Similarities: Contains 1 PAC (PAS-associated C-terminal) domain.

Poids moléculaire: 15 kDa

ID gène: 5216

UniProt: P07737

Pathways: Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location