HSP70 1A anticorps (C-Term)

N° du produit ABIN3043849

Cet anticorps Lapin Polyclonal détecte spécifiquement HSP70 1A dans WB, IHC, IF, ICC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 13+ publications.

Aperçu rapide pour HSP70 1A anticorps (C-Term) (ABIN3043849)

Antigène: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSP70 1A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-HSP70 1A anticorps

Épitope: C-Term

Fonction: Anti-Hsp70/HSPA1A/HSPA1B Antibody

Séquence: KGKISEADKK KVLDKCQEVI SWLDANTLAE KDEFEHKR

Specificité: No cross reactivity with other proteins.

Réactivité croisée (Details): No cross-reactivity with other proteins

Homologie: different from the related mouse sequence by five amino acids,and from the related rat sequence by three amino acids.

Attributs du produit: Anti-Hsp70/HSPA1A/HSPA1B Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-HSP70 1A (ABIN3043849)

Zeng, Xiao, Deng, Li: "Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8985623, (2017) (PubMed).

Zhang, Tang, Li, Li, Zhang, Yan, Cui: "Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5371-8, (2016) (PubMed).

Chen, Wan, Huang, Wang, Liu, Wang: "Study on the immune response to recombinant Hsp70 protein from Megalobrama amblycephala." dans: Immunobiology, Vol. 219, Issue 11, pp. 850-8, (2014) (PubMed).

Jing, Liu, Chen, Hu, Xiao, Ma: "Acute effect of copper and cadmium exposure on the expression of heat shock protein 70 in the Cyprinidae fish Tanichthys albonubes." dans: Chemosphere, Vol. 91, Issue 8, pp. 1113-22, (2013) (PubMed).

Pu, Shi, Wang, Wang, Guo, Liu, Sun, Gao, Zhou: "Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats." dans: Journal of biomedical research, Vol. 25, Issue 4, pp. 292-8, (2013) (PubMed).

Chen, Zuo, Yang, Lu, Wang, Wang, Tu, Chen, Liu, Liu, Xiao: "Severe multiple organ injury in HSF1 knockout mice induced by lipopolysaccharide is associated with an increase in neutrophil infiltration and surface expression of adhesion molecules." dans: Journal of leukocyte biology, Vol. 92, Issue 4, pp. 851-7, (2012) (PubMed).

Li, Roubeix, Wang, Shi, Liu, Baudouin, Chen: "Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system." dans: Molecular vision, Vol. 18, pp. 317-29, (2012) (PubMed).

Fan, Jiang, Yang, Liu, Song, Pan: "Effects of adrenergic agents on stress-induced brain microstructural and immunochemical changes in adult male Wistar rats." dans: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, Vol. 193, Issue 5, pp. 418-24, (2011) (PubMed).

Xu, Luo, Wu, Chen, Xu, Gao, He, Geng, Xiao, Zhang, Chen, Li: "Immunohistological evaluation of skin responses after treatment using a fractional ultrapulse carbon dioxide laser on back skin." dans: Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], Vol. 37, Issue 8, pp. 1141-9, (2011) (PubMed).

Ouyang, Gao, Wang, Xie, Lei, Zhou, Liao, Zhong, Tang: "Thermoseed hyperthermia treatment of mammary orthotopic transplantation tumors in rats and impact on immune function." dans: Oncology reports, Vol. 24, Issue 4, pp. 973-9, (2010) (PubMed).

Yi, Peng, Chang, Peng, Yan, Lin: "Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells." dans: World journal of gastroenterology, Vol. 13, Issue 15, pp. 2174-8, (2007) (PubMed).

Wu, Cao, Gao, Chen, Wang, Zumbika, Qian: "Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells." dans: World journal of gastroenterology, Vol. 10, Issue 20, pp. 2944-8, (2004) (PubMed).

Gnatyshak, Loginskiĭ, Mazurok: "[Homo- and autotransplantation of bone marrow in radiation and chemotherapy of oncologic patients]." dans: Vrachebnoe delo, Vol. 4, pp. 129-32, (1973) (PubMed).

Détails sur HSP70 1A

Antigène: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Autre désignation: HSPA1A

Sujet:

Background: HSPA1 (heat shock 70 kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al. (1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Gene Full Name: Heat shock 70 kDa protein 1A

Sequence Similarities: Contains 1 HMG box DNA-binding domain.

Poids moléculaire: 70 kDa

ID gène: 3303, 3304

UniProt: P0DMV8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process