Versican anticorps (N-Term)

N° du produit ABIN3043957

Cet anticorps Lapin Polyclonal détecte spécifiquement Versican dans WB et IHC. Il présente une réactivité envers Souris, Humain et Rat et a été mentionné dans 1 publication.

Aperçu rapide pour Versican anticorps (N-Term) (ABIN3043957)

Antigène: Versican (Vcan)

Reactivité: Souris, Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Versican est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Versican anticorps

Épitope: AA 65-92, N-Term

Fonction: Anti-Versican/VCAN Antibody Picoband®

Séquence: KWSKIEVDKN GKDLKETTVL VAQNGNIK

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Versican/VCAN Antibody Picoband® (ABIN3043957). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Bode-Lesniewska, B., Dours-Zimmermann, M. T., Odermatt, B. F., Briner, J., Heitz, P. U., Zimmermann, D. R. Distribution of the large aggregating proteoglycan versican in adult human tissues. J. Histochem. Cytochem. 44: 303-312, 1996. 2. Dours-Zimmermann, M. T., Zimmermann, D. R. A novel glycosaminoglycan attachment domain identified in two alternative splice variants of human versican. J. Biol. Chem. 269: 32992-32998, 1994. 3. Naso, M. F., Morgan, J. L., Buchberg, A. M., Siracusa, L. D., Iozzo, R. V. Expression pattern and mapping of the murine versican gene (Cspg2) to chromosome 13. Genomics 29: 297-300, 1995.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Versican (ABIN3043957)

Fujii, Karpova, Asagoe, Georgiev, Dummer, Urosevic-Maiwald: "Versican upregulation in Sézary cells alters growth, motility and resistance to chemotherapy." dans: Leukemia, Vol. 29, Issue 10, pp. 2024-32, (2015) (PubMed).

Détails sur Versican

Antigène: Versican (Vcan)

Autre désignation: VCAN

Sujet:

Synonyms: Versican core protein,Chondroitin sulfate proteoglycan core protein 2,Chondroitin sulfate proteoglycan 2,Glial hyaluronate-binding protein,GHAP,Large fibroblast proteoglycan,PG-M,VCAN,CSPG2,

Tissue Specificity: Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.

Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000 kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Sequence Similarities: Belongs to the aggrecan/versican proteoglycan family.

Poids moléculaire: 373 kDa

ID gène: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process