Versican anticorps (N-Term)

N° du produit ABIN3043957

Détail du produit anti-Versican anticorps

Antigène: Versican (Vcan)

Épitope: AA 65-92, N-Term

Reactivité: Souris, Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Versican est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Versican core protein(VCAN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Séquence: KWSKIEVDKN GKDLKETTVL VAQNGNIK

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Versican core protein(VCAN) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: versican
Protein Name: Versican core protein

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human Versican (65-92aa KWSKIEVDKNGKDLKETTVLVAQNGNIK), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human , The detection limit for Versican is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Références pour anticorps anti-Versican (ABIN3043957)

Fujii, Karpova, Asagoe, Georgiev, Dummer, Urosevic-Maiwald: "Versican upregulation in Sézary cells alters growth, motility and resistance to chemotherapy." dans: Leukemia, Vol. 29, Issue 10, pp. 2024-32, (2015) (PubMed).

Détails sur Versican

Antigène: Versican (Vcan)

Autre désignation: VCAN (Vcan Produits)

Synonymes: anticorps br146, anticorps cspg2, anticorps cspg2a, anticorps sb:cb63, anticorps versican, anticorps CSPG2, anticorps Cspg2, anticorps wgn, anticorps ervr, anticorps pg-m, anticorps wgn1, anticorps Xversican, anticorps dcan, anticorps cspg2b, anticorps fb99b07, anticorps wu:fb99b07, anticorps ERVR, anticorps GHAP, anticorps PG-M, anticorps WGN, anticorps WGN1, anticorps 5430420N07Rik, anticorps 9430051N09, anticorps DPEAAE, anticorps NG2, anticorps PG-M(V0), anticorps PG-M(V1), anticorps hdf, anticorps versican a, anticorps versican, anticorps versican b, anticorps vcana, anticorps VCAN, anticorps Vcan, anticorps vcan, anticorps vcanb

Sujet: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000 kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Synonyms: Chondroitin sulfate proteoglycan 2 antibody|Chondroitin sulfate proteoglycan core protein 2 antibody|CSPG2 antibody|CSPG2_HUMAN antibody|ERVR antibody|GHAP antibody|Glial hyaluronate binding protein antibody|Glial hyaluronate-binding protein antibody|Large fibroblast proteoglycan antibody|PG-M antibody|PGM antibody|VCAN antibody|Versican core protein antibody|Versican proteoglycan antibody|WGN 1 antibody|WGN antibody|WGN1 antibody

ID gène: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process