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DDX4 anticorps (Middle Region)

DDX4 Reactivité: Humain, Souris, Rat WB, IHC (p), IHC (fro), ICC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN3044032
  • Antigène Voir toutes DDX4 Anticorps
    DDX4 (DEAD Box Protein 4 (DDX4))
    Épitope
    • 16
    • 7
    • 7
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 253-272, Middle Region
    Reactivité
    • 52
    • 25
    • 16
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 67
    • 8
    Lapin
    Clonalité
    • 69
    • 6
    Polyclonal
    Conjugué
    • 29
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp DDX4 est non-conjugé
    Application
    • 53
    • 25
    • 16
    • 13
    • 13
    • 10
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Fonction
    Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Séquence
    EDEDSIFAHY QTGINFDKYD
    Réactivité croisée (Details)
    No cross reactivity with other proteins.
    Attributs du produit
    Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Gene Name: DEAD(Asp-Glu-Ala-Asp) box polypeptide 4
    Protein Name: Probable ATP-dependent RNA helicase DDX4
    Purification
    Immunogen affinity purified.
    Immunogène
    A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.
    Isotype
    IgG
    Top Product
    Discover our top product DDX4 Anticorps primaire
  • Indications d'application
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for DDX4 is approximately 0.5 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Commentaires

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Agent conservateur
    Thimerosal (Merthiolate), Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Date de péremption
    12 months
  • Antigène
    DDX4 (DEAD Box Protein 4 (DDX4))
    Autre désignation
    DDX4 (DDX4 Produits)
    Synonymes
    anticorps VASA, anticorps AV206478, anticorps Mvh, anticorps RVLG, anticorps vasa, anticorps vlg1, anticorps DEAD-box helicase 4, anticorps DEAD (Asp-Glu-Ala-Asp) box polypeptide 4, anticorps DEAD-box helicase 4 L homeolog, anticorps DDX4, anticorps Ddx4, anticorps ddx4.L
    Sujet
    DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

    Synonyms: DDX 4 antibody|Ddx4 antibody|DDX4_HUMAN antibody|DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody|DEAD box protein 4 antibody|Dead-box protein 4 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody|MGC111074 antibody|MVH antibody|Probable ATP dependent RNA helicase DDX4 antibody|Probable ATP-dependent RNA helicase DDX4 antibody|VASA antibody|VASA homolog antibody
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