NOX1 anticorps (Middle Region)

N° du produit ABIN3044251

Cet anticorps Lapin Polyclonal détecte spécifiquement NOX1 dans WB et IHC. Il présente une réactivité avec des échantillons de Rat et Souris. Il a été cité dans 3+ publications.

Aperçu rapide pour NOX1 anticorps (Middle Region) (ABIN3044251)

Antigène: NOX1 (NADPH Oxidase 1 (NOX1))

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NOX1 anticorps

Épitope: AA 417-431, Middle Region

Fonction: Anti-NADPH oxidase 1 NOX1 Antibody

Séquence: WYKFQRAHNK LKTQK

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-NADPH oxidase 1 NOX1 Antibody (ABIN3044251). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of rat NOX1, different from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-NOX1 (ABIN3044251)

Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." dans: Antioxidants & redox signaling, (2018) (PubMed).

Wang, Wang, Liu, Wang, Zhao, Wang, Yin: "Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells." dans: Carbohydrate polymers, Vol. 125, pp. 135-45, (2015) (PubMed).

Gu, Gong, Zhang, Dong, Zhao, Burczynski, Wang, Sun, Zhu, Han, Wang, Li: "Regulation of transforming growth factor beta 1 gene expression by dihydropteridine reductase in kidney 293T cells." dans: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 91, Issue 3, pp. 187-93, (2013) (PubMed).

Détails sur NOX1

Antigène: NOX1 (NADPH Oxidase 1 (NOX1))

Autre désignation: NOX1

Sujet:

Synonyms: NADPH oxidase 1,NOX-1,1.-.-.-,Mitogenic oxidase 1,MOX-1,NADH/NADPH mitogenic oxidase subunit P65-MOX,NOH-1,Nox1,Mox1, Noh1,

Tissue Specificity: Expressed in vascular smooth muscle cells.

Background: NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58 % identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50 % survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Sequence Similarities: Contains 1 FAD-binding FR-type domain.

Poids moléculaire: 65 kDa

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport