PEBP1 anticorps (N-Term)

N° du produit ABIN3044276

Détail du produit anti-PEBP1 anticorps

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Épitope: AA 15-29, N-Term

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PEBP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse,Rat.

Séquence: QEVDEPPQHA LRVDY

Réactivité croisée (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Attributs du produit: Rabbit IgG polyclonal antibody for Phosphatidylethanolamine-binding protein 1(PEBP1) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: phosphatidylethanolamine binding protein 1
Protein Name: Phosphatidylethanolamine-binding protein 1(PEBP-1)

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of mouse PBP(15-29aa QEVDEPPQHALRVDY), identical to the related rat sequence.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur PEBP1

Antigène: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Autre désignation: PEBP1 (PEBP1 Produits)

Synonymes: anticorps HCNP, anticorps HCNPpp, anticorps PBP, anticorps PEBP, anticorps PEBP-1, anticorps RKIP, anticorps pbp, anticorps hcnp, anticorps pebp, anticorps rkip, anticorps Pbp, anticorps Pbp1, anticorps Pbpr, anticorps Rkip, anticorps PEBP1, anticorps BcDNA:LP12095, anticorps CG18594, anticorps Dmel\\CG18594, anticorps T2, anticorps zgc:56033, anticorps zgc:76942, anticorps phosphatidylethanolamine binding protein 1, anticorps phosphatidylethanolamine binding protein 1 L homeolog, anticorps phosphatidylethanolamine-binding protein, anticorps phosphatidylethanolamine-binding protein,putative, anticorps Phosphatidylethanolamine-binding protein 1, anticorps PEBP1, anticorps pebp1, anticorps Pebp1, anticorps pebp1.L, anticorps RR_RS07785, anticorps PVX_235290, anticorps PVX_123630, anticorps PKH_143640, anticorps EAM_1207

Sujet: PEBP1(Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein(PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al(2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

Synonyms: HCNP antibody|HCNPpp antibody|Hippocampal cholinergic neurostimulating peptide antibody|Neuropolypeptide h3 antibody|PEBP antibody|PEBP 1 antibody|PEBP-1 antibody|PEBP1 antibody|PEBP1_HUMAN antibody|Phosphatidylethanolamine binding protein antibody|Phosphatidylethanolamine binding protein 1 antibody|Prostatic binding protein antibody|Prostatic-binding protein antibody|R kip antibody|Raf kinase inhibitor protein antibody|Raf kinase inhibitory protein antibody|RKIP antibody

UniProt: P70296

Pathways: Feeding Behaviour