PMVK anticorps (Middle Region)

N° du produit ABIN3044328

Cet anticorps anti-PMVK est un anticorps Lapin Polyclonal détectant PMVK dans WB et IHC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour PMVK anticorps (Middle Region) (ABIN3044328)

Antigène: PMVK (Phosphomevalonate Kinase (PMVK))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PMVK est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PMVK anticorps

Épitope: AA 135-151, Middle Region

Fonction: Anti-Phosphomevalonate kinase PMVK Antibody Picoband®

Séquence: EQSRQQRGWV FTPGVDD

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Phosphomevalonate kinase PMVK Antibody (ABIN3044328). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human PMVK, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Human, Mouse
Western blot, 0.1-0.5 μg/mL, Rat, Human, Mouse
1. Olivier, L. M., Chambliss, K. L., Gibson, K. M., Krisans, S. K. : Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting. J. Lipid Res. 40: 672-679, 1999. 2. Chambliss, K. L., Slaughter, C. A., Schreiner, R., Hoffmann, G. F., Gibson, K. M. : Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence. J. Biol. Chem. 271: 17330-17334, 1996.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PMVK

Antigène: PMVK (Phosphomevalonate Kinase (PMVK))

Autre désignation: PMVK

Sujet:

Synonyms: Phosphomevalonate kinase,PMKase,hPMK,2.7.4.2,PMVK,PMKI,

Tissue Specificity: Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung. .

Background: PMVK (phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence (PTS-1), Ser-Arg-Leu, at the C terminus of the protein.

Poids moléculaire: 77 kDa

UniProt: Q15126