AQP3 anticorps (C-Term)

N° du produit ABIN3044413

Cet anticorps Lapin Polyclonal détecte spécifiquement AQP3 dans WB, IHC, IF, ICC et FACS. Il présente une réactivité envers Humain, Rat et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour AQP3 anticorps (C-Term) (ABIN3044413)

Antigène: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AQP3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-AQP3 anticorps

Épitope: AA 278-292, C-Term

Fonction: Anti-Aquaporin 3/AQP3 Antibody Picoband®

Séquence: EENVKLAHVK HKEQI

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Aquaporin 3/AQP3 Antibody (ABIN3044413). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Dibas AI, Mia AJ, Yorio T (1998). "Aquaporins (water channels): role in vasopressin-activated water transport". Proc. Soc. Exp. Biol. Med. 219 (3): 183-99. 2. Roudier N, Ripoche P, Gane P, Le Pennec PY, Daniels G, Cartron JP, Bailly P (2002). "AQP3 deficiency in humans and the molecular basis of a novel blood group system, GIL". J. Biol. Chem. 277 (48): 45854-9. 3. Sasaki S, Ishibashi K, Marumo F (1998). "Aquaporin-2 and -3: representatives of two subgroups of the aquaporin family colocalized in the kidney collecting duct". Annu. Rev. Physiol. 60: 199-220.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-AQP3 (ABIN3044413)

Shin, Lee, Gil, Kim, Choe, Kim, Lee, Lim: "Agerarin, identified from Ageratum houstonianum, stimulates circadian CLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes." dans: Scientific reports, Vol. 7, Issue 1, pp. 11175, (2017) (PubMed).

Wang, Bu, Zhang, Chen, Zhang, Bao: "Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema." dans: Molecular medicine reports, Vol. 12, Issue 4, pp. 5625-32, (2016) (PubMed).

Détails sur AQP3

Antigène: AQP3 (Aquaporin 3 (Gill Blood Group) (AQP3))

Autre désignation: AQP3

Sujet:

Synonyms: Aquaporin-3,AQP-3,Aquaglyceroporin-3,AQP3,

Tissue Specificity: Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.

Background: This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Sequence Similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family.

Poids moléculaire: 32-36 kDa

UniProt: Q92482