Fatty Acid Synthase anticorps (AA 1-226)

N° du produit ABIN3044520

Cet anticorps anti-Fatty Acid Synthase est un anticorps Lapin Polyclonal détectant Fatty Acid Synthase dans WB, IHC, IF et ICC. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Fatty Acid Synthase anticorps (AA 1-226) (ABIN3044520)

Antigène: Fatty Acid Synthase (FASN)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fatty Acid Synthase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Fatty Acid Synthase anticorps

Épitope: AA 1-226

Fonction: Anti-Fatty Acid Synthase/FASN Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Fatty Acid Synthase/FASN Antibody Picoband® (ABIN3044520). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunofluorescence, 5 μg/mL
1. "Entrez Gene: FASN fatty acid synthase". 2. Jayakumar A, Tai MH, Huang WY, al-Feel W, Hsu M, Abu-Elheiga L, Chirala SS, Wakil SJ (Oct 1995). "Human fatty acid synthase: properties and molecular cloning". Proc Natl Acad Sci U S A 92 (19): 8695-9. 3. Smith S, Witkowski A, Joshi AK (July 2003). "Structural and functional organization of the animal fatty acid synthase". Prog. Lipid Res. 42 (4): 289-317.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Fatty Acid Synthase (ABIN3044520)

Lu, Ma, Cai: "Increased HAGLR expression promotes non-small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis." dans: Tumour biology, Vol. 39, Issue 4, pp. 1010428317697570, (2017) (PubMed).

Détails sur Fatty Acid Synthase

Antigène: Fatty Acid Synthase (FASN)

Autre désignation: FASN

Sujet:

Synonyms: Fatty acid synthase;2.3.1.85;[Acyl-carrier-protein] S-acetyltransferase;2.3.1.38;[Acyl-carrier-protein] S-malonyltransferase;2.3.1.39;3-oxoacyl-[acyl-carrier-protein] synthase;2.3.1.41;3-oxoacyl-[acyl-carrier-protein] reductase;1.1.1.100;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;4.2.1.59;Enoyl-[acyl-carrier-protein] reductase;1.3.1.39;Oleoyl-[acyl-carrier-protein] hydrolase;3.1.2.14;FASN;FAS;

Tissue Specificity: Ubiquitous. Prominent expression in brain, lung, and liver. .

Background: Fatty acid synthase  (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Poids moléculaire: 273 kDa

ID gène: 2194

UniProt: P49327

Pathways: AMPK Signaling