SMYD3 anticorps (C-Term)

N° du produit ABIN3044548

Cet anticorps Lapin Polyclonal détecte spécifiquement SMYD3 dans WB et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour SMYD3 anticorps (C-Term) (ABIN3044548)

Antigène: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMYD3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SMYD3 anticorps

Épitope: AA 388-428, C-Term

Fonction: Anti-SMYD3 Antibody Picoband®

Séquence: QAMKNLRLAF DIMRVTHGRE HSLIEDLILL LEECDANIRA S

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SMYD3 Antibody Picoband® (ABIN3044548). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human SMYD3, different from the related mouse sequence by one amino acid.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Hamamoto, R., Furukawa, Y., Morita, M., Iimura, Y., Silva, F. P., Li, M., Yagyu, R., Nakamura, Y. SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells. Nature Cell Biol. 6: 731-740, 2004. 2. Mazur, P. K., Reynoird, N., Khatri, P., Jansen, P. W. T. C., Wilkinson, A. W., Liu, S., Barbash, O., Van Aller, G. S., Huddleston, M., Dhanak, D., Tummino, P. J., Kruger, R. G., Garcia, B. A., Butte, A. J., Vermeulen, M., Sage, J., Gozani, O. SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer. Nature 510: 283-287, 2014. 3. Tsuge, M., Hamamoto, R., Silva, F. P., Ohnishi, Y., Chayama, K., Kamatani, N., Furukawa, Y., Nakamura, Y. A variable number of tandem repeats polymorphism in an E2F-1 binding element in the 5-prime flanking region of SMYD3 is a risk factor for human cancers. Nature Genet. 37: 1104-1107, 2005.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur SMYD3

Antigène: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Autre désignation: SMYD3

Sujet:

Synonyms: Histone-lysine N-methyltransferase SMYD3,2.1.1.43,SET and MYND domain-containing protein 3,Zinc finger MYND domain-containing protein 1,SMYD3,ZMYND1, ZNFN3A1,

Tissue Specificity: Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. .

Background: SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene. The International Radiation Hybrid Mapping Consortium mapped the SMYD3 gene to chromosome 1. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 55 kDa

ID gène: 64754

UniProt: Q9H7B4