SKP2 anticorps (N-Term)

N° du produit ABIN3044574

Cet anticorps anti-SKP2 est un anticorps Lapin Polyclonal détectant SKP2 dans WB et IHC. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 7+ publications.

Aperçu rapide pour SKP2 anticorps (N-Term) (ABIN3044574)

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SKP2 anticorps

Épitope: AA 2-20, N-Term

Fonction: Anti-SKP2 Antibody Picoband®

Séquence: HRKHLQEIPD LSSNVATSF

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-SKP2 Antibody (ABIN3044574). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human SKP2, different from the related rat and mouse sequences by three amino acids.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Kitagawa, M., Lee, S. H., McCormick, F. : Skp2 suppresses p53-dependent apoptosis by inhibiting p300. Molec. Cell 29: 217-231, 2008. 2. Latres, E., Chiarle, R., Schulman, B. A., Pavletich, N. P., Pellicer, A., Inghirami, G., Pagano, M. : Role of the F-box protein Skp2 in lymphomagenesis. Proc. Nat. Acad. Sci. 98: 2515-2520, 2001.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Agent conservateur: Thimerosal (Merthiolate), Sodium azide

Précaution d'utilisation: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-SKP2 (ABIN3044574)

Chen, Xie, Zhao, Wang, Bai, Yin, Jiang, Xie, Jia, Huang et al.: "Ampelopsin induces apoptosis by regulating multiple c?Myc/S?phase kinase?associated protein 2/F?box and WD repeat?containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma ..." dans: Molecular medicine reports, Vol. 11, Issue 1, pp. 105-12, (2014) (PubMed).

Xu, Zhu, Han, You, Liu, Ye, Zhang, Wang, Yao: "The oncoprotein hepatitis B X-interacting protein promotes the migration of ovarian cancer cells through the upregulation of S-phase kinase-associated protein 2 by Sp1." dans: International journal of oncology, Vol. 45, Issue 1, pp. 255-63, (2014) (PubMed).

Chen, Bai, Zhong, Xie, Long, Yang, Wu, Jia, Wang: "Wogonin has multiple anti-cancer effects by regulating c-Myc/SKP2/Fbw7? and HDAC1/HDAC2 pathways and inducing apoptosis in human lung adenocarcinoma cell line A549." dans: PLoS ONE, Vol. 8, Issue 11, pp. e79201, (2013) (PubMed).

Qiu, Zhang, Lv, Zhang, Zhu, Wang, Yao: "Cdh1 inhibits reactive astrocyte proliferation after oxygen-glucose deprivation and reperfusion." dans: Neurochemistry international, Vol. 63, Issue 2, pp. 87-92, (2013) (PubMed).

Xu, You, Liu, Shen, Yao, Ye, Zhang: "The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells." dans: Cancer letters, Vol. 333, Issue 1, pp. 124-32, (2013) (PubMed).

Su, Wang, Qi, Zhao, Li, Cui: "Small interfering RNA targeting of S phase kinase-interacting protein 2 inhibits cell proliferation of pterygium fibroblasts." dans: Molecular vision, Vol. 17, pp. 247-56, (2011) (PubMed).

Su, Wang, Yan, Teng, Cui: "Inhibition of proliferation of rabbit lens epithelial cells by S-phase kinase-interacting protein 2 targeting small interfering RNA." dans: Molecular vision, Vol. 16, pp. 907-15, (2010) (PubMed).

Détails sur SKP2

Antigène: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Autre désignation: SKP2

Sujet:

Synonyms: S-phase kinase-associated protein 2,Cyclin-A/CDK2-associated protein p45,F-box protein Skp2,F-box/LRR-repeat protein 1,p45skp2,SKP2,FBXL1,

Tissue Specificity: Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. .

Background: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1)-S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.

Sequence Similarities: Contains 1 F-box domain.

Poids moléculaire: 25 kDa

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases