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His Tag anticorps (FITC)

Name: His Tag anticorps (FITC)
SKU: ABIN304882
Price: 860.5 EUR
Availability: InStock

IHC, FACS Hôte: Souris Monoclonal AD1-1-10 FITC

Image no. 1 for anti-His Tag antibody (FITC) (ABIN304882)

1 image

N° du produit ABIN304882

Fiche de données

Antigène Voir toutes His Tag Anticorps

His Tag

Reactivité

Veuillez nous consulter SVP
Hôte
93

56

12

9

8

7

2

1
Souris
Anticorps secondaires appropriés
Clonalité
106

69

10
Monoclonal
Conjugué
91

23

13

12

4

3

3

2

2

2

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2

2

2

2

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Cet anticorp His Tag est conjugé à/à la FITC
Application
153

103

38

28

24

16

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

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Immunohistochemistry (IHC), Flow Cytometry (FACS)

Séquence

HHHHHH

Specificité

Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Purification

Protein G purified

Immunogène

PAX6 transcription factor linked to histidine tag.

Type of Immunogen: Fusion protein

Clone

AD1-1-10

Isotype

IgG1

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WB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2 HRP

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Reactivité: Humain WB Hôte: Lapin Polyclonal RB19960 unconjugated

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WB, ELISA Hôte: Lapin Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2 Biotin

Indications d'application

Approved: Flo (1:10), IHC

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 0.09 % sodium azide, 1 % BSA.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

Stock

4 °C,-20 °C

Stockage commentaire

Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Antigène

His Tag

Abstract

His Tag Produits

Classe de substances

Tag