His Tag anticorps (FITC)

N° du produit ABIN304882

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans IHC et FACS.

Aperçu rapide pour His Tag anticorps (FITC) (ABIN304882)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: AD1-1-10

Détail du produit anti-His Tag anticorps (FITC)

Séquence: HHHHHH

Specificité: Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.

Purification: Protein G purified

Immunogène: PAX6 transcription factor linked to histidine tag.

Type of Immunogen: Fusion protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo (1:10), IHC

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 1 % BSA.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.

Détails sur His Tag

Antigène: His Tag

Classe de substances: Tag