ATG12 anticorps (Internal Region)

N° du produit ABIN305747

L’anticorps anti-ATG12 Polyclonal Lapin est utilisé pour la détection de ATG12 dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC, ELISA, IP et IHC (p).

Aperçu rapide pour ATG12 anticorps (Internal Region) (ABIN305747)

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG12 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ATG12 anticorps

Épitope: Internal Region

Specificité: Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from the internal region of the human Apg12 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 7 μg/reaction.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur ATG12

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Autre désignation: APG12 / ATG12

Sujet: Name/Gene ID: ATG12

Synonyms: ATG12, Autophagy-related protein 12, APG12L, Autophagy related 12, FBR93, APG12, APG12-like, Ubiquitin-like protein ATG12, HAPG12

ID gène: 9140

UniProt: O94817

Pathways: Autophagy