Claudin 12 anticorps (N-Term)

N° du produit ABIN305786

L’anticorps Lapin Polyclonal anti-Claudin 12 a été validé pour WB, IHC, IHC (p) et IF. Il convient pour détecter Claudin 12 dans des échantillons de Humain et Souris.

Aperçu rapide pour Claudin 12 anticorps (N-Term) (ABIN305786)

Antigène: Claudin 12 (CLDN12)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Claudin 12 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-Claudin 12 anticorps

Épitope: N-Term

Marque: IHC-plus™

Specificité: Recognizes mouse Claudin 12 protein at ~27kD in Western blots. Cross-reactivity has been confirmed on mouse lung, colon and kidney homogenates. Recognizes human protein in IHC-P.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from the N-terminal region of mouse and human Claudin 12.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 5 μg/mL, performed using ethanol-fixed mouse colon. Positive control: Mouse lung, colon, and kidney homogenates.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Claudin 12

Antigène: Claudin 12 (CLDN12)

Autre désignation: CLDN12 / Claudin 12

Sujet: Name/Gene ID: CLDN12
Family: Claudin

Synonyms: CLDN12, Claudin 12, Claudin-12

ID gène: 9069

UniProt: P56749

Pathways: Cell-Cell Junction Organization