TJP2 anticorps (C-Term)

N° du produit ABIN305886

Cet anticorps anti-TJP2 est un anticorps Lapin Polyclonal détectant TJP2 dans WB, IHC, IF et IHC (p). Adapté pour Humain, Souris, Rat et Chien.

Aperçu rapide pour TJP2 anticorps (C-Term) (ABIN305886)

Antigène: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TJP2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TJP2 anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from the C-terminal region of human or canine ZO-2.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IF (2.5 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunofluorescence: 2.5 μg/mL. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays. Positive control: Western Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C, Long term: aliquot to Avoid freeze-thaw cycles, and store at -20°C.

Détails sur TJP2

Antigène: TJP2 (Tight Junction Protein 2 (Zona Occludens 2) (TJP2))

Autre désignation: TJP2 / ZO2 / ZO-2

Sujet: Name/Gene ID: TJP2
Family: Occludin

Synonyms: TJP2, C9DUPq21.11, DUP9q21.11, DFNA51, ZO-2, Zona occludens 2, Zonula occludens protein 2, Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104, Zona occludens protein 2

ID gène: 9414

UniProt: Q9UDY2