Occludin anticorps (C-Term)

N° du produit ABIN305888

L’anticorps Lapin Polyclonal anti-Occludin a été validé pour WB, IHC, ELISA, IP, IF et IHC (p). Il convient pour détecter Occludin dans des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour Occludin anticorps (C-Term) (ABIN305888)

Antigène: Occludin (OCLN)

Reactivité: Humain, Souris, Rat, Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Occludin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Occludin anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.

Purification: Immunoaffinity purified

Immunogène: A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.

Type of Immunogen: Fusion protein

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Occludin

Antigène: Occludin (OCLN)

Autre désignation: OCLN / Occludin

Sujet: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

ID gène: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C