GFAP anticorps (AA 417-430)

N° du produit ABIN305893

Cet anticorps Chèvre Polyclonal détecte spécifiquement GFAP dans WB, IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain, Rat, Boeuf (Vache), Lapin, Singe, Cheval et Roussette (Chauve-souris).

Aperçu rapide pour GFAP anticorps (AA 417-430) (ABIN305893)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain, Rat, Boeuf (Vache), Lapin, Singe, Cheval, Roussette (Chauve-souris)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GFAP anticorps

Épitope: AA 417-430

Specificité: Human GFAP.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bat, Bovine, Elephant, Horse, Rabbit (100%) Mouse, Dog, Pig, Turkey, Chicken (93%) Opossum, Lizard (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-DGEVIKESKQEHKD from the C-terminus of human GFAP (NP_002046.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bat, Bovine, Elephant, Horse, Rabbit (100%), Mouse, Dog, Pig, Turkey, Chicken (93%), Opossum, Lizard (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet: Name/Gene ID: GFAP

Synonyms: GFAP

ID gène: 2670

NCBI Accession: NP_002046

UniProt: P14136