Phosphotyrosine anticorps (Peroxidase (POD))

N° du produit ABIN306703

Cet anticorps Souris Monoclonal détecte spécifiquement Phosphotyrosine dans WB, IP, IHC et EIA.

Aperçu rapide pour Phosphotyrosine anticorps (Peroxidase (POD)) (ABIN306703)

Antigène: Phosphotyrosine

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Phosphotyrosine est conjugé à/à la Peroxidase (POD)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Clone: PY20

Détail du produit anti-Phosphotyrosine anticorps (Peroxidase (POD))

Fonction: Mouse anti Phosphotyrosine, conjugated with peroxidase

Attributs du produit: The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The peroxidase conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce undesirable background signals.

Purification: Prot A/G

Immunogène: Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Isotype: IgG2b

Information d'application

Indications d'application: Application: A dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunoprecipitation, immunohistochemistry, or immunocytochemistry.

Restrictions: For Research Use only

Stockage

Buffer: 60 % glycerol, phosphate buffered saline, and 0.1 % 2-Chloroacetamide

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur Phosphotyrosine

Antigène: Phosphotyrosine

Classe de substances: Amino Acid

Sujet: Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate